争霸8官方入口

争霸8官方入口:生物科学与工程争霸8官方入口

College of Biological Science and Engineering

林娟

发布日期:2022-07-07 发布者:

姓名:林娟

性别:女

职称:教授,博导

学历:博士

电话:0591-22866376

电子邮件:ljuan@fzu.edu.cn

研究方向:合成生物学,生物催化与酶分子工程,细胞工厂

教育工作经历:

1988.9-1992.7:华东师范大学生物系,大学本科;

1998.9-2001.7:争霸8官方入口生物化工专业,硕士研究生;

2003.9-2007.4:争霸8官方入口分析化学专业,博士研究生;

1992.7-1995.4:福建农林大学;

1995.5-今:争霸8官方入口生物科学与工程争霸8官方入口.

教学简介:

争霸8官方入口教学名师。近几年承担了中央财政支持地方高校发展专项资金项目《生物与食品学科研究生创新创业实践基地建设》,争霸8官方入口教改项目《微生物学实验教学改革》、《微生物学双语教学》、《细胞生物学网络课程建设》、《细胞生物学精品课程建设》、《进一步完善我校本科生导师制的研究》、《微生物学系列课程教学团队建设》、《生物工程类本科生创新创业实践平台建设》10多项教改课题的研究,在高等教育出版社出版的《高校生命科学基础课程报告论坛文集》、《争霸8官方入口学报》(哲社版)、《高等教育研究》、《高教论坛》、《教育教学论坛》等刊物上发表教改论文10余篇。在2003、2004年的争霸8官方入口第五届、第六届青年教师“最佳一节课”竞赛活动中,分别获得优秀奖和二等奖的好成绩,并获得福建省教学成果二等奖1项、争霸8官方入口教学成果奖4项,7次获得争霸8官方入口教学优秀二等奖,2015年获得争霸8官方入口厦航奖教金。主讲的《微生物学》和《细胞生物学》课程分别被评为福建省精品课程、争霸8官方入口核心课程和争霸8官方入口精品课程。

科研简介:

福建省高等学校新世纪优秀人才支持计划人选。主要从事合成生物学,生物催化与酶分子工程,工业微生物代谢工程改造。主持了国家863计划子课题、国家海洋局海洋公益性行业科研专项子课题、福建省高校产学科技重大项目、福建省海洋高新产业发展专项、福建省经信委企业技术创新项目、福建省发改委生物产业专项、福建省自然科学基金重点项目、福建省科技厅重点项目以及产学研等30多项科研课题的研究工作;以第一作者或通讯作者发表SCI文章60多授权国家发明专利18件。

社会兼职:

中国生物化学与分子生物学会酶学专业分会委员,福建省生物化学与分子生物学会副理事长;福建省微生物学会常务理事;福建省水产学会理事。

承担的主要科研项目:

1、 白藜芦醇生物合成技术开发,横向项目,2024-2027;

2、 5-羟基色氨酸及褪黑素的生物合成技术开发,横向项目,2024-2026;

3、 D-对甲砜基苯丝氨酸乙酯(D-乙酯)的产业化生产,横向项目,2022-2026;

4、 甾醇类化合物酶催化技术开发,横向项目,2022-2025;

5、 工业属性高效生物催化剂的设计与开发,横向项目,2019-2023;

6、 生物发酵功能饲料的开发,横向项目,2019-2022;

7、 福建省科技创新重点项目D-对甲砜基苯丝氨酸乙酯的生物催化新技术开发及产业化生产”,2022-2025;

8、 福建省基金重点项目“环己酮单加氧酶不对称氧化奥美拉唑硫醚的分子机理研究”,2021-2024;

9、 福建省高校产学合作重大项目“高色价红曲红色素液态深层发酵关键技术开发”,2017-2020;

10、 福建省经信委企业技术创新项目“海洋藻类资源高值化利用技术创新平台建设”,2015-2017;

11、 福建省海洋高新产业发展专项“鱼罐头加工废弃液的高值化利用”,2015-2017

12、 国家科技部863计划子课题“新型糖酶创制与创新应用关键技术研究”,2013-2017;

13、 国家海洋局海洋公益性行业科研专项子课题“海洋生物酶资源库及几种海洋源性工业用酶关键生产技术开发”,2013-2016;

14、 福建省高校产学合作重大项目“利用现代微生物工程技术发酵生产超氧化物歧化酶”,2013-2016;

15、 福建省发改委生物产业项目“漆酶的产业化生产技术开发”,2011-2014。


以第一作者或通讯作者发表的主要论文:

1. Multienzymatic Cascade for Synthesis of Hydroxytyrosol via TwoStage Biocatalysis. Journal of Agricultural and Food Chemistry, 2024.

2. Single-cell enzymatic cascade synthesis of testolactone enabled by engineering of polycyclic ketone monooxygenase and multi-gene expression fine-tuning. International Journal of Biological Macromolecules, 2024.

3. Advances in Enzymatic Incorporation of Small Fluorine Modules. European Journal Organic Chemistry, 2024.

4. An artificial biocatalytic cascade for efficient synthesis of norepinephrine by combination of engineered L-threonine transaldolase with multi-enzyme expression fine-tuning. International Journal of Biological Macromolecules, 2024.

5. Development of whole cell biocatalytic system for asymmetric synthesis of esomeprazole with enhancing coenzyme biosynthesis pathway. Enzyme and Microbial Technology, 2024.

6. Efficiently manufacturing ectoine via metabolic engineering and protein engineering of L-2,4-diaminobutyrate transaminase. International Journal of Biological Macromolecules, 2024.

7. Immobilization of laccase on mesoporous metal organic frameworks for efcient crosscoupling of ethyl ferulate. World Journal of Microbiology and Biotechnology, 2024.

8. Rational design of short‐chain dehydrogenase/reductase for enantio‐complementary synthesis of chiral 1,2‐diols by successive hydroxymethylation and reduction of aldehydes. Biotechnology and Bioengineering, 2024.

9. Rational design on loop regions for precisely regulating flexibility of catalytic center to mitigate overoxidation of prazole sulfides by Baeyer-Villiger monooxygenase. Bioorganic Chemistry, 2024.

10. Batch and continuous flow asymmetric synthesis of anabolic-androgenic steroids via a single-cell biocatalytic Δ1 -dehydrogenation and C17β-carbonyl reduction cascade. Green Chemistry, 2023.

11. A chemoenzymatic process for preparation of highly purified dehydroepiandrosterone in high space-time yield. Bioorganic Chemistry, 2023.

12. A chemoenzymatic strategy for the efficient synthesis of amphenicol antibiotic chloramphenicol mediated by an engineered L-threonine transaldolase with high activity and stereoselectivity, Catalysis Science & Technology, 2023.

13. Efficient synthesis of 2’-deoxyguanosine in one-pot cascade by employing an engineered purine nucleoside phosphorylase from Brevibacterium acetylicum. World Journal of Microbiology and Biotechnology, 2023.

14. Engineering of carbonyl reductase for asymmetric reduction of difficult-to-reduce ketone tetrahydrofuran-3-one. Molecular Catalysis, 2023.

15. Immobilization of laccase on magnetic nanoparticles for enhanced polymerization of phenols. Enzyme and Microbial Technology, 2023.

16. Improved Antimicrobial Activity of Bovine Lactoferrin Peptide (LFcinB) Based on Rational Design. The Protein Journal, 2023.

17. Multiplex metabolic pathway engineering of Monascus pilosus enhances lovastatin production. Applied Microbiology and Biotechnology, 2023.

18. Construction of an Efficient Non-natural Enzyme System for Preparation of Testosterone in High Space-Time Yield. ACS Sustainable Chemistry & Engineering, 2022.

19. Focused mutagenesis in non-catalytic cavity for improving catalytic efficiency of -ketosteroid -Δ1-dehydrogenase, Molecular Catalysis, 2022.

20. “Nonpolarity paving” in substrate tunnel of a Limnobacter sp. Phenylacetone monooxygenase for efficient single whole-cell synthesis of esomeprazole. Bioorganic Chemistry, 2022.

21. An Effective Chemo-Enzymatic method with An Evolved L-Threonine Aldolase for Preparing L-threo-4-Methylsulfonylphenylserine Ethyl Ester of High Optical Purity. Molecular Catalysis, 2022.

22. Engineered Cyclohexylamine Oxidase with Improved Activity and Stereoselectivity for Asymmetric Synthesis of a Bulky Dextromethorphan Precursor and Its Analogues. ChemCatChem, 2022.

23. Construction of efficient enzyme systems for preparing chiral ethyl 3-hydroxy- 3 -phenylpropionate. Enzyme and Microbial Technology, 2022.

24. Efficient biosynthesis of (2S, 3R)-4-methylsulfonylphenylserine by artificial self-assembly of enzyme complex combined with an intensified acetaldehyde elimination system. Bioorganic Chemistry, 2021.

25. Improving the Cβ Stereoselectivity of l-Threonine Aldolase for the Synthesis of l-threo-4-Methylsulfonylphenylserine by Modulating the Substrate-Binding Pocket To Control the Orientation of the Substrate Entrance. Chemistry—A European Journal, 2021.

26. Design of a PL18 alginate lyase with flexible loops and broader entrance to enhance the activity and thermostability. Enzyme and Microbial Technology, 2021.

27. Single-Point Mutation Near Active Center Increases Substrate Affinity of Alginate Lyase AlgL-CD. Applied Biochemistry and Biotechnology, 2021.

28. Multi-enzyme cascade for improving ?-hydroxy-a-amino acids production by engineering L- threonine transaldolase and combining acetaldehyde elimination system. Bioresource Technology, 2020.

29. Asymmetric Synthesis of a Key Dextromethorphan Intermediate and Its Analogs Enabled by a New Cyclohexylamine Oxidase: Enzyme Discovery, Reaction Development, and Mechanistic Insight. Journal of Organic Chemistry, 2020.

30. A L-threonine aldolase for asymmetric synthesis of β-hydroxy-α-amino acids. Chemical Engineering Science, 2020.

31. Expression and characterization of a chitinase from Serratia marcescens. Protein Expression and Puri?cation, 2020.

32. Rational Design of Dehydrogenase/Reductases Based on Comparative Structural Analysis of Prereaction-State and Free-State Simulations for E?cient Asymmetric Reduction of Bulky Aryl Ketones. ACS Catalysis, 2019.

33. Characteristics of L-threonine transaldolase for asymmetric synthesis of β-hydroxy-α-amino acids. Catalysis Science & Technology, 2019.

34. Truncation of κ?carrageenase for higher κ?carrageenan oligosaccharides yield with improved enzymatic characteristics. International Journal of Biological Macromolecules, 2019.

35. Mutagenesis on the surface of a β-agarase from Vibrio sp. ZC-1 increased its thermo-stability. Enzyme and Microbial Technology, 2019.

36. Inhibitory mechanism of Penicillin V on mushroom tyrosinase. Molecular Biology Reports, 2019.

37. Prediction of zinc-binding sites using multiple sequence profiles and machine learning methods. Molecular Omics, 2019.

38. Preparation of bioactive neoagaroligosaccharides through hydrolysis of Gracilaria lemaneiformis agar: A comparative study. Food Chemistry, 2018.

39. A Novel Multi-domain High Molecular, Salt-Stable Alkaline Xylanase from Alkalibacterium sp. SL3. Frontiers in Microbiology, 2017.

40. Inactivation kinetics and conformation change of Hypocrea orientalis b-glucosidase with guanidine hydrochloride. Journal of Bioscience and Bioengineering, 2017.

41. Degradation of tetracycline by immobilized laccase and the proposed transformation pathway. Journal of Hazardous Materials, 2017.

42. Immobilized Cerrena sp. Laccase preparation, thermal inactivation, and operational stability in malachite green decolorization. Scientific Reports, 2017.

43. Laccases Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation. Frontiers in Microbiology, 2017.

44. A novel cold-adapted and highly salt-tolerant esterase from Alkalibacterium sp. SL3 from the sediment of a soda lake. Scientific Reports, 2016.

45. Cross-linked enzyme aggregates of Cerrena laccase: Preparation, enhanced NaCl tolerance and decolorization of Remazol Brilliant Blue Reactive. Journal of the Taiwan Institute of Chemical Engineers, 2016.

46. Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with fungal laccase. Analytical Biochemistry, 2016.

47. Laccase Production and Differential Transcription of Laccase Genes in Cerrena sp.in Response to Metal Ions, Aromatic Compounds, and Nutrients. Frontiers in Microbiology, 2016.

48. Optimal parameters for laccase-mediated destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels. Data in Brief, 2016.

49. A neural network learning approach for improving the prediction of residue depth based on sequencederived features. RSC Advances, 2016.

50. Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis. Molecules, 2016.

51. Highly efficient expression and characterization of a β-mannanase from Bacillus subtilis in Pichia pastoris. Biotechnology and Applied Biochemistry, 2016.

52. The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Biochem Biophys Res Commun, 2016.

53. Laccase-Catalyzed Decolorization of Malachite Green: Performance Optimization and Degradation Mechanism. PLOS ONE, 2015.

54. Molecular characterization of a thermophilic and salt and alkaline-tolerant xylanase from Planococcus sp. SL4, a strain isolated from the sediment of a soda lake. Journal of Microbiology and BiotechnologyJMB), 2015.

55. Highly efficient expression and characterization of a β-mannanase from Bacillus subtilis in Pichia pastoris, Biotechnology and Applied Biochemistry, 2015.

56. A novel laccase from basidiomycete Cerrena sp.: Cloning, eterologousexpression, and characterization. International Journal of Biological Macromolecules, 2015.

57. Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing Ability. PLOS ONE, 2014.

58. High Phylogenetic Diversity of Glycosyl Hydrolase Family 10 and 11 Xylanases in the Sediment of Lake Dabusu in China. PLOS ONE, 2014.

59. Antioxidant activities of kombucha prepared from three different substrates and changes in content of probiotics during storage. Food Science and Technology, 2014.

获奖情况:

教学:分别获得争霸8官方入口第五届、第六届青年教师“最佳一节课”竞赛优秀奖和二等奖,并获得福建省教学成果二等奖1项,争霸8官方入口教学成果奖4项,7次获得校教学优秀二等奖,2015年获得争霸8官方入口厦航奖教金。

科研:获省科技进步三等奖1项,2007年入选福建省高等学校新世纪优秀人才支持计划。


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